About us
Production of Antibodies
Producing monoclonal mouse antibodies at Abfrontier involves several key steps. Our Monoclonal antibodies are Korean laboratory-made molecules that can mimic the immune system's ability to fight off harmful pathogens such as viruses and bacteria. Here is an overview of the production process:
Antigen Selection:
Identify and select the target antigen against which the antibodies will be generated. The antigen is a specific molecule that triggers an immune response.
Immunization:
Inject mice with the chosen antigen to stimulate an immune response. The immune system of the mice will produce antibodies against the antigen.
Cell Fusion:
Isolate B cells from the immunized mice. B cells are responsible for producing antibodies. These B cells are then fused with immortalized myeloma cells to create hybridoma cells. Hybridomas have the ability to continuously produce antibodies.
Screening and Cloning:
Select hybridoma cells that produce antibodies with high specificity and affinity for the target antigen. Screening is typically done using techniques like enzyme-linked immunosorbent assay (ELISA).
Expansion of Hybridoma Cells:
Culture and expand the selected hybridoma cells in a suitable growth medium. This step is crucial to produce a sufficient quantity of monoclonal antibodies.
Antibody Purification:
Harvest the culture supernatant containing the antibodies and purify the antibodies to remove any contaminants. Common purification methods include chromatography.
Testing and Characterization:
Characterize the purified monoclonal antibodies for specificity, affinity, and functionality. Various assays are used to assess the binding properties and biological activity of the antibodies.
Scaling Up Production:
Once a viable hybridoma cell line is established and characterized, scale up the production to generate larger quantities of monoclonal antibodies.
Storage:
Store the purified antibodies without freeze thawing to maintain for stability.
Distributors
Belgium |
+32 2 265 09 20 |
Voortstraat 49, 1910 Kampenhout |
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Netherlands |
+31 20 808 0893 |
Kuiper 1, 5521 DG Eersel The Netherlands |
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Germany |
+49 0241 93688188 |
Marienbongard 20, 52062 Aachen |
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Spain |
+ 34 0911 87 65 58 |
Avenida de AndalucÃa, 27, 29006 Málaga |
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France |
+33 01 43 25 01 50 |
9 Rue Lagrange, 75005 Paris |
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Italy |
+39 0350086186 |
Piazza Giacomo Matteotti n.6, 24122 Bergamo |
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Poland |
+48 058 710 33 44 |
lok.2, Grunwaldzka 88A, 81-771 Sopot |
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Bulgaria |
+359 089 745 5388 |
Ul iSkar 53, 1191 Kokalyane |
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UK |
+44 020 3393 8531 |
Station Close, Unicorn House, Potters Bar EN6 1TL |
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USA |
+1 4087800908 |
6017 Snell Avenue, San Jose, CA 95123 |
Antibody team
Tony Fred, Biologist
Founder of our lab with 7 years of Lab experience.
Mich Stark, COO
Mich loves orking with mice. With his multi-year experience as Lab Technician, Mich has helped the company to get where it is today. Mich is among the best minds.
Aline Turner, CTO
Aline is one aughter of the Turner Biosystems family. She mentors customer support on flow antibodies.
Iris Joe, CFO
Iris, with her international experience, helps us easily understand the ustomers questions to bring the company to the next level.